Accurate observation of the development of a chick embryo and analyzing the details of its formation necessarily needs a microscope. Microscope is very essential and significant in this kind of study and observation. The suitable specimen for embryonic study is the chick embryo. Several procedures are being observed while preparing chick embryos. These are the following procedures:
1. Prepare a finger bowl and fill it with three quarters of normal saline solution heated under an incubation temperature of 103 degrees Fahrenheit. Remove an egg from an incubator and strike it sharply against the edge of the finger bowl to crack. Insert a thumbnail into the crack and pull gently the two halves of the egg apart. Hold the half shell with contents of the egg using the right hand.
2. Hold the shell section with the egg contents over an empty finger bowl and slightly tip the shell to allow as much of the egg white as possible in order to drain off into the finger bowl.
3. Immerse the shell gently and its contents into the warm saline solution in the other finger bowl. To permit the yolk to slide gently into the liquid saline, tilt the shell.
4. The yolk will float in the saline solution with the embryo side up. If the embryo is not on top of the yolk, gently roll it over to the proper position with a fingertip.
5. Carefully cut the embryo from the yolk using a pair of scissors. The disk shaped central mass is made while the floating yolk is steadied with the flat side of the pair of forceps in the free hand.
6. The embryo blastoderm adheres to the vitelline membrane, a thin sheet of vascular tissue in which the embryos blood vessels are located. Grasp the edge of the membrane in the forceps to remove the embryo. Do this while slipping a watch glass into the saline solution and gradually positioning the glass under the floating blastoderm. Pull up the watch glass and its contents out of the solution, and transfer it to the worktable.


